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Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters - Preparation of methyl esters of fatty acids has-hardcopy to reduce data amount to1 Scope This document specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely: a) a rapid transmethylation procedure under alkaline conditions; b) a general transmethylation methylation procedure under sequential alkaline and acid conditions;