Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g By omitting Folic acid and

By omitting Folic acid and Riboflavine from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium

5 Na2HPO4 0

0 Beef Extract 5

Tryptone 10 NaCl 8 Agar 15 Final pH 7

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g By omitting Folic acid andBoric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room