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Description

Terrific BrothTerrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E. coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium. Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid. Tryptone and Yeast are present in the medium in elevated concentrations

3 KH2PO4 6

C6H12O6 180

The antibiotic inhibits crosslinking of peptidoglycan by binding and inactivation of transpeptidases

CAS 31282-04-9

the “dropped out” supplements

Tryptone 20 Yeast extract 5 NaCl 0

03 Suspend 74

44 Agar 15 Final pH 7

The concentration of NaCl in LB Miller is twice the concentration of the LB Lennox Broth formulation and twenty times the concentration of LB Luria Broth formulation

Besides these components

9% pH 1% Di HB2BO 3

Aspartic acid is added

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