Name: Lee's Medium
Brand: martinafruit.com
SKU: 80193058571
Price: 158.25 USD
Availability: InStock
Rating: 4.4833 (30 reviews)

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Description

Lee's MediumFormulation (NH4)2SO4 5. 0 g L MgSO4. 7H2O 0. 2 g L K2HPO 2. 5 g L NaCl 5. 0 g L L Alanine 0. 5 g L L Leucine 1. 3 g L L Lysine 1. 0 g L L Methionine 0. 1 g L L Ornithine 0. 0714 g L L Phenylalanine 0. 5 g L L Proline 0. 5 g L L Threonine 0. 5 g L Biotin 0. 001 g L Suspend 17. 09g in 1L of Distilled or De ionised Water HYGROSCOPIC Store at Room Temperature DO NOT BREATHE DUST Keep Container Tightly Closed

2 at 25°C Suspend 16 gram powdered medium in 1 litre distilled water

Lysine and Methionine

2 at 25°C Suspend 15

1kg pack size

10 g/L pH - 4

2 Glucose 20 Tween 80 1 Sodium Acetate 5 Dipotassium Hydrogen Phosphate 2 Manganese sulphate 0

Threonine are slightly increased as well in SIH

Glucose 11000 g/l KH2PO4 680

0 L-Threonine 47

2 g/L K2HPO 2

For propagation of of lambda phages in Escherichia coli

at this strength the ph is around 6