Protein L - 50nm Gold NanoUrchins Size:0.5ML

Protein L - 50nm Gold NanoUrchins Size:0.5MLGold nanoparticle core size: 50nm Absorbance (lambda max.): 560nm Optical density: Available at OD=3 and OD=10, select in product options Conjugated protein: Recombinant Protein L, from P. magnus produced in E. coli Working dilution: 1: 1 1: 100 Supplied in: 0. 5XPBS (pH 7. 4), 20% glycerol (v v), 1% BSA Applications Protein L gold nanourchin conjugates are suitable for use in immunoblotting, light microscopy, and electron microscopy applications.

Concentration: OD=50 (when dissolved to a final volume of 100ul)

(M-1cm-1) Size Dispersity (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/Volume Ratio Particle Mass (g) Molar Mass (g/mol) Molar Concentration* 100 572 3

darkfield microscopy

This kit can also be used for determining if conjugation was successful of an antigen to the gold nanoparticles if probed with an antibody from the list above (not including in kit) prior to running the test

5 maleimide groups/nm2) (5kDa PEG-spacer between gold surface and maleimide-group)

can be used immediately or allow the plate to dry and store appropriately for future use

): 400-410nm

Conjugated antibody: Affinity purified anti-mouse IgG F(ab')2 fragment

Absorption max: 410nm

Side-scatter histogram of 90nm size reference gold nanoparticles

Composition: Methylated (methoxy-PEG-thiol

Conjugated antibody: Affinity purified anti-human IgG (H+L)