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Soil quality. Laboratory methods for determination of microbial soil respiration Ingestion-build-159183 BS 7494-2 also guides you1 Scope This International Standard describes methods for the determination of soil microbial respiration of aerobic, unsaturated soils. The methods are suitable for the determination of O2 uptake or CO2 release, either after addition of a substrate (substrate induced respiration), or without substrate addition (basal respiration). This International Standard is applicable to the measurement of soil respiration in order to: determine the microbial